The later cut-off time means M&S has extended the deadline by three hours since the start of the year when it was 8pm.
Stores where the two-hour proposition is being tested include Cheshire Oaks and London Oxford Street.
The 11pm cut-off reflects the fact that although many M&S shoppers typically browse between 7pm and 10pm, 5% of all orders are made between 10pm and 11pm.
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